High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System

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• 作者：Anselm P. Moshi^a ; ^b ; ^{ansemoshi@yahoo.co.uk" class="auth_mail} ; Carla F. Crespo^a ; ¹ ; ^{carlacrespo123@gmail.com" class="auth_mail} ; Malik Badshah^a ; ² ; ^{malikbadshah@gmail.com" class="auth_mail} ; Kenneth M.M. Hosea^b ; ^{kenhosea2@gmail.com" class="auth_mail} ; Anthony Manoni Mshandete^b ; ^{anthony.mshandete@yahoo.com" class="auth_mail} ; Bo Mattiasson^a ; ^{Bo.Mattiasson@biotek.lu.se" class="auth_mail}
• 关键词：Manihot glaziovii ; Fed-batch ; Bioethanol ; Automatic Gas Potential Test System ; Simultaneous saccharification and fermentation
• 刊名：Bioresource Technology
• 出版年：March, 2014
• 年：2014
• 卷：156
• 期：Complete
• 页码：348-356
• 全文大小：1221 K

文摘

A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava <em>Manihot glazioviiem>. FB-SSF allowed fermentation of up to 390 g/L of starch-derived glucose achieving high bioethanol concentration of up to 190 g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava <em>M. glazioviiem> starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl庐 and AMG庐). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO₂ registered in the AMPTS software correlated positively with samples analysed by HPLC (<em>Rem>² = 0.99).