Release of Growth Factors into Root Canal by Irrigations in Regenerative Endodontics

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• 作者：Qian Zeng ; PhD^&lowast ; ; Sean Nguyen ; DMD^&dagger ; ; Hongming Zhang ; PhD^&dagger ; ; Hari Priya Chebrolu ; BDS ; MDS ; DMD^&dagger ; ; Dalia Alzebdeh ; PhD^&Dagger ; ; Mustafa A. Badi ; DDS ; MS^§ ; ; Jong Ryul Kim ; DMD ; MSD ; PhD^&dagger ; ; Junqi Ling ; PhD^|| ; ^{lingjq@mail.sysu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Maobin Yang ; DMD ; MDS ; PhD^&dagger ; ; ^{myang@temple.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cell migration ; growth factors ; irrigation ; regenerative endodontics ; root canal surface
• 刊名：Journal of Endodontics
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：42
• 期：12
• 页码：1760-1766
• 全文大小：1224 K

文摘

The aim of this study was to investigate the release of growth factors into root canal space after the irrigation procedure of regenerative endodontic procedure.

Methods

Sixty standardized root segments were prepared from extracted single-root teeth. Nail varnish was applied to all surfaces except the root canal surface. Root segments were irrigated with 1.5% NaOCl + 17% EDTA, 2.5% NaOCl + 17% EDTA, 17% EDTA, or deionized water. The profile of growth factors that were released after irrigation was studied by growth factor array. Enzyme-linked immunosorbent assay was used to validate the release of transforming growth factor (TGF)-β1 and basic fibroblast growth factor (bFGF) at 4 hours, 1 day, and 3 days after irrigation. The final concentrations were calculated on the basis of the root canal volume measured by cone-beam computed tomography. Dental pulp stem cell migration on growth factors released from root segments was measured by using Transwell assay.

Results

Total of 11 of 41 growth factors were detected by growth factors array. Enzyme-linked immunosorbent assay showed that TGF-β1 was released in all irrigation groups. Compared with the group with 17% EDTA (6.92 ± 4.49 ng/mL), the groups with 1.5% NaOCl + 17% EDTA and 2.5% NaOCl + 17% EDTA had significantly higher release of TGF-β1 (69.04 ± 30.41 ng/mL and 59.26 ± 3.37 ng/mL, respectively), with a peak release at day 1. The release of bFGF was detected at a low level in all groups (0 ng/mL to 0.43 ± 0.22 ng/mL). Migration assay showed the growth factors released from root segments induced dental pulp stem cell migration.

Conclusions

The root segment model in present study simulated clinical scenario and indicated that the current irrigation protocol released a significant amount of TGF-β1 but not bFGF. The growth factors released into root canal space induced dental pulp stem cell migration.