Complete sequence of Fig fleck-associated virus, a novel member of the family Tymoviridae

详细信息    查看全文

• 作者：Toufic  ; Elbeaino^a ;   ; ^{elbeaino@iamb.it} ; Michele  ; Digiaro^a ; Giovanni P.  ; Martelli^b
• 关键词：Ficus carica ; FFkaV ; Tymoviridae ; Cloning and sequencing ; PCR detection ; Distribution
• 刊名：Virus Research
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：161
• 期：2
• 页码：198-202
• 全文大小：467 K

文摘

The complete nucleotide sequence and the genome organization were determined of a novel virus, tentatively named Fig fleck-associated virus (FFkaV). The viral genome is a positive-sense, single-stranded RNA 7046 nucleotides in size excluding the 3?terminal poly(A) tract, and comprising two open reading frames. ORF1 encodes a polypeptide of 2161 amino acids (p240), which contains the signatures of replication-associated proteins and the coat protein cistron (p24) at its 3?end. ORF2 codes for a 461 amino acid protein (p50) identified as a putative movement proteins (MP). In phylogenetic trees constructed with sequences of the putative polymerase and CP proteins FFkaV consistently groups with members of the genus Maculavirus, family Tymoviridae. However, the genome organization diverges from that of the two completely sequenced maculaviruses, Grapevine fleck virus (GFkV) and Bombix mori Macula-like virus (BmMLV), as it exhibits a structure resembling that of Maize rayado fino virus (MRFV), the type species of the genus Marafivirus and of Olive latent virus 3 (OLV-3), an unclassified virus in the family Tymoviridae. FFkaV was found in field-grown figs from six Mediterranean countries with an incidence ranging from 15 % to 25 % .