Propagation of field highly pathogenic porcine reproductive and respiratory syndrome virus in MARC-145 cells is promoted by cell apoptosis
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- 作者：Mengyun Ge ; Yi Zhang ; Ying Liu ; Tao Liu ; Fanya Zeng ; zengfanya@scu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Field HP-PRRSV ; Cell-to-cell transmission ; Infectious cell cluster ; Apoptosis ; MARC-145 cells
- 刊名：Virus Research
- 出版年：2016
- 出版时间：2 February 2016
- 年：2016
- 卷：213
- 期：Complete
- 页码：322-331
- 全文大小：4100 K
文摘
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Propagation of field HP-PRRSV in MARC-145 is cell-density dependent and the propagation is more effective in overgrown cells.
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Apoptosis triggered automatically in the overgrown MARC-145 cells promotes the propagation of field virus in these cells.
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Induction of apoptosis in MARC-145 cells mimics the promotion and further increased viral production.
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A vigorous viral RNA replication and fast virus release in early life cycle of viral infection were detected in the apoptosis induced cells.
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Propagation of wild type PRRSV in apoptosis induced cells may provide a way to replicate the virus for clinical diagnosis and vaccine development.