Investigation on mycoplasma populations in pneumonic dairy lamb lungs using a DNA microarray assay

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• 作者：Marco Bottinelli^a ; Christiane Schnee^b ; Elvio Lepri^a ; Valentina Stefanetti^a ; Giovanni Filippini^c ; Marco Gobbi^c ; Martina Sebastianelli^c ; Pietro Antenucci^d ; Elisa Rampacci^a ; Mauro Coletti^a ; Fabrizio Passamonti^a ; ^{fabrizio.passamonti@unipg.it}
• 关键词：Mycoplasma ; Microarray ; Lambs ; Respiratory
• 刊名：Small Ruminant Research
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：147
• 期：Complete
• 页码：96-100
• 全文大小：666 K
• 卷排序：147

文摘

An epidemiological survey on the Mycoplasma species living in pneumonic lungs of young dairy lambs in Italy was carried out. A culture-independent approach, a DNA microarray assay for Mycoplasma species, was used together with other PCR assays targeting other respiratory pathogens. Prevalence was 21.7% for M. ovipneumonie and 5.2% for M. arginini. A statistically significant positive correlation between co-presence/absence of Mycoplasma spp. and Mannheimia haemolytica was found. No correlation was found between lung lesion degree and positivity for Mycoplasma.