Detection of premature stop codons leading to truncated internalin A among food and clinical strains of Listeria monocytogenes

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• 作者：Margarida Ferreira da Silva^a ; ^b ; ^a38491@ua.pt ; Vâ ; nia Ferreira^a ; ^{vferreira@porto.ucp.pt} ; Rui Magalhã ; es^a ; ^{rmagalhaes@porto.ucp.pt} ; Gonç ; alo Almeida^a ; ^{galmeida@porto.ucp.pt} ; Artur Alves^b ; ^{artur.alves@ua.pt} ; Paula Teixeira^a ; ^{pcteixeira@porto.ucp.pt}
• 关键词：Listeria monocytogenes ; inlA ; Internalin A ; Premature stop codons (PMSCs) ; Caco-2 ; Invasion assay
• 刊名：Food Microbiology
• 出版年：2017
• 出版时间：May 2017
• 年：2017
• 卷：63
• 期：Complete
• 页码：6-11
• 全文大小：398 K
• 卷排序：63

文摘

22 isolates analysed for mutations in inlA leading to premature stop codons (PMSCs). Three food-associated and one clinical isolates presented PMSCs in inlA. Isolates with PMSCs in inlA showed reduced invasion efficiency in Caco-2 cell line. One clinical isolate showed attenuated invasion phenotype but a full-length InlA.