Characterisation of capillary ionic liquid columns for gas chromatography-mass spectrometry analysis of fatty acid methyl esters
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- 作者：Annie Xu Zeng ; Sung-Tong Chin ; Yada Nolvachai ; Chadin Kulsing ; Leonard M. Sidisky ; Philip J. Marriott
- 关键词：Ionic liquid phases ; High resolution gas chromatography ; Fatty acid separation ; Equivalent chain length ; Abraham descriptors
- 刊名：Analytica Chimica Acta
- 出版年：25 November, 2013
- 年：2013
- 卷：803
- 期：Complete
- 页码：166-173
- 全文大小：1348 K
文摘
Due to their distinct chemical properties, the application of ionic liquid (IL) compounds as gas chromatography (GC) stationary phases offer unique GC separation especially in the analysis of geometric and positional fatty acid methyl ester (FAME) isomers. Elution behaviour of FAME on several commercialised IL capillary columns including phosphonium based SLB-IL59, SLB-IL60, SLB-IL61 and SLB-IL76 and imidazolium based SLB-IL82, SLB-IL100, and SLB-IL111 as well as a general purpose column SLB-5ms, were evaluated in gas chromatography-mass spectrometry (GC-MS) analysis. The phases were further characterised by using a linear solvation energy relationship (LSER) approach according to the equivalent chain length (ECL) index of FAME. Among all tested IL columns, elution temperatures of saturated FAME increased as their McReynolds鈥?polarity value decreased, except for IL60. ECL values increased markedly as the stationary phase polarity increased, particularly for the polyunsaturated FAME. The LSER study indicated a lowest l/e value at 0.864 for IL111, displaying phase selectivity towards unsaturated FAME, with higher peak capacity within a carbon number isomer group. s and e descriptors calculated from LSER were validated by excellent correlation with dipole moments and lowest unoccupied molecular orbital (LUMO) energies, with R2 values of 0.99 and 0.92 respectively, calculated using GAUSSIAN.