Subnanogram Detection of Silver Stained Protein Bands with Thermal Lens Spectrometry

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• 作者：Giulia Mazza ; ^{giulia.mazza@donau-uni.ac.at} ; Thomas Posnicek ; Lisa-Marie Wagner ; Martin Brandl
• 关键词：Thermal lens spectrometry ; gel electrophoresis ; protein detection
• 刊名：Procedia Engineering
• 出版年：2016
• 出版时间：2016
• 年：2016
• 卷：168
• 期：Complete
• 页码：602-605
• 全文大小：302 K
• 卷排序：168

文摘

Sodium dodecyl sulfate polyacrylamide gel electrophoresis is a standard biochemical technique to separate, detect and quantify protein amounts of specific molecular weights. Colorimetric detection with Coomassie or silver stain is by far the most common analysis method. Silver stain is the most sensitive one and protein bands down to 1 ng can be detected with laboratory imaging systems. To increase the sensitivity of the detection we developed a sensor based on the principle of the thermal lens effect to scan, record, and quantify protein bands not visible to the eye or to the camera. Our device could detect bands of 0.05 ng in gels soaked in methanol/water and 0.1 ng when soaked in water.