T24 HRAS transformed NIH/3T3 mouse cells (GhrasT-NIH/3T3) in serial tumorigenic in vitro/in vivo passages give rise to increasingly aggressive tumorigenic cell lines T1-A and T2-A and metastatic cell lines T3-HA and T4-PA
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- 作者：Durwood B. Raya ; b ; raydb@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Gerald A. Merrilla ; 1 ; Merrilljer@aol.com" class="auth_mail" title="E-mail the corresponding author ; Frederic J. Brennera ; BrennerFJ@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Laurie S. Lytlea ; LSLytle@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Tan Lamb ; 2 ; tanmlam@aol.com" class="auth_mail" title="E-mail the corresponding author ; Aaron McElhinneya ; 3 ; aaron.mcelhinney@msn.com" class="auth_mail" title="E-mail the corresponding author ; Joel Andersa ; 4 ; jba6776@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Tara Tauber Rocka ; 5 ; taramtauber@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Jennifer Kier Lykera ; 6 ; jenlyker@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Scott Barcusa ; 7 ; scott.barcus@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Kara Hust Lesliea ; 8 ; karaleslie31@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Jill M. Kramera ; 9 ; jkramer@buffalo.edu" class="auth_mail" title="E-mail the corresponding author ; Eric M. Rubensteina ; 10 ; emrubenstein@bsu.edu" class="auth_mail" title="E-mail the corresponding author ; Karen Pryor Schanza ; 11 ; kkschanz@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Amy J. Parkhursta ; 12 ; amyjparkhurst@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Michelle Pecka ; 13 ; Peckmichellea@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Kimberly Gooda ; 14 ; kimberlyross@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Kristi Lemke Granatha ; 15 ; granathkl@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Nicole Cifraa ; 16 ; cifranm1@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Jessalee Wantz Detweilera ; 17 ; mattnjessd@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Laura Stevensa ; 18 ; laura.stevens2011@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Richard Albertsona ; 19 ; rma165@psu.edu" class="auth_mail" title="E-mail the corresponding author ; Rachael Deira ; 20 ; rd41193n@pace.edu" class="auth_mail" title="E-mail the corresponding author ; Elisabeth Stewarta ; 21 ; elisabeth.e.stewart@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Katherine Wingarda ; 22 ; wingardkl1@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Micah Rose Richardsona ; RichardsonMR1@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Sarah B. Blizarda ; BlizardSB1@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Lauren E. Gillespiea ; GillespieLE1@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Charles E. Krileya ; cekrieley@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; Daniel I. Rzewnickia ; Rzewnickidi1@gcc.edu" class="auth_mail" title="E-mail the corresponding author ; David H. Jonesa ; b ; 23 ; j.jeandave@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：NIH/3T3 ; HRAS ; Tansformed ; GhrasT-NIH/3T3 ; In vitro/in vivo tumorigenic progression ; Metastasis ; Punctuated evolution ; Chromoplexy ; Chromothripsis ; Doubling time method
- 刊名：Experimental Cell Research
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：340
- 期：1
- 页码：1-11
- 全文大小：1624 K
文摘
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A tumor progression model derived from a new T24 HRAS transformed NIH/3T3 cell line.
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T1-A, T2-A, T3-HA, T4PA lines derived from GhrasT-NIH/3T3, T1-A, T2-A, T3-HA tumors.
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PCR detected the human HRAS oncogene in T2-A and T4-PA metastatic tumors.
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These five new cell lines showed a progressive increase in tumorigenic potential.
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New method obtained doubling times using daily photomicrographs of gridded plates.