Possible involvement of Hcn1 ion channel in learning and memory dysfunction in SAMP8 mice
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- 作者：Maruf Mohammad Akbor ; Koji Tomobe ; Tomomi Yamada ; Juhyon Kim ; Hiroki Mano ; Nobuyuki Kurosawa ; Kazuo Sasaki ; Yasuyuki Nomura ; Masaharu Isobe
- 关键词：SAMP8 ; Chromosome 13 ; QTL analysis ; RNA-seq analysis ; Learning and memory deficiency ; Hcn1
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：8 November, 2013
- 年：2013
- 卷：441
- 期：1
- 页码：25-30
- 全文大小：990 K
文摘
The senescence-accelerated mouse prone 8 (SAMP8) strain exhibits age-related learning and memory deficits (LMD) at 2 months of age. Combined linkage analysis of 264 F2 intercross SAMP8 脳 JF1 mice and RNA-seq analysis identified Hcn1 gene out of 29 genes in the LMD region on chromosome 13. Hcn1 in SAMP8 strain showed 15 times less polyglutamine repetition compared to Japanese fancy mouse 1 (JF1). Whole cell patch clamp analysis showed that Hcn1 ion conductivity was significantly lower in SAMP8 compared to that of JF1, which may be associated with learning and memory deficiency.