dl-3-n-Butylphthalide attenuation of methamphetamine-induced neurotoxicity in SH-SY5Y neuroblastoma cells

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• 作者：Jun Zhao^a ; ^b ; Jianuo Liu^c ; Enquan Xu^c ; Yunlin Liu^b ; Anmu Xie^a ; ^{xieanmu@163.com" class="auth_mail" title="E-mail the corresponding author} ; Huangui Xiong^c ; ^{hxiong@unmc.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Methamphetamine ; dl-3-n-Butylphthalide ; Neurotoxicity ; Neuroprotection
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 November 2016
• 年：2016
• 卷：165
• 期：Complete
• 页码：16-20
• 全文大小：677 K

文摘

Methamphetamine (Meth) abuse causes neural injury in the brain. There are no efficacious therapies available to treat Meth-induce neural injury. The present study intended to test the therapeutic potential of dl-3-n-butylphthalide (NBP), a chemical compound extracted originally from the seeds of Chinese Celery, in the amelioration and prevention of Meth-induced neural injury.

d="absSec_2">Main methods

d="sp0025">Experiments were carried out on SH-SY5Y cells. Neuronal injury and apoptotic cell death were detected by MTT assay and analysis of nuclear morphology. Intracellular reactive oxygen species (ROS) was evaluated by dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay and protein expression levels of the apoptosis-related cleaved caspase-3, bcl2 and Bax were determined by western blotting.

d="absSec_3">Key findings

d="sp0030">Treatment of SH-SY5Y cells with Meth induced cell injury and apoptosis. NBP attenuated Meth-associated cell injury and apoptosis via blockage of Meth-mediated upregulation of intracellular ROS production and inhibition of Meth-induced decrease of cleaved caspase-3/caspase-3 and Bcl-2/Bax ratios.

d="absSec_4">Significance

d="sp0035">The results presented in this study indicate that NBP may have therapeutic benefits in the treatment of Meth-induced neuronal injury in the central nervous system.