Insulin dependent apolipoprotein B degradation and phosphatidylinositide 3-kinase activation with microsomal translocation are restored in McArdle RH7777 cells following serum deprivation
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- 作者：Janet D. Sparks ; Janet_Sparks@urmc.rochester.edu" class="auth_mail" title="E-mail the corresponding author ; Amy L. Magra ; Jeffrey M. Chamberlain ; Colleen O'Dell ; Charles E. Sparks
- 关键词：Apo B ; PI3K ; IRS1 ; IRS2 ; PTP1B ; VLDL ; Liver
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2016
- 出版时间：8 January 2016
- 年：2016
- 卷：469
- 期：2
- 页码：326-331
- 全文大小：535 K
文摘
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Enriched culture conditions increase apo B secretion by McA cells.
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Insulin induced Class I PI3K activity is blunted in McA cells under enriched conditions.
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IDAD sensitive McA cells demonstrate insulin-dependent pY-IRS1, but not pY-IRS2, translocation to intracellular membranes.
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RH and McA cells resistant to IDAD express increased PTP1B.
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Inhibition of protein tyrosine phosphatases including PTP1B is sufficient to restore IDAD in insulin resistant McA cells.