miR-154 inhibits prostate cancer cell proliferation by targeting CCND2

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• 作者：Chen Zhu ; Pengfei Shao ; Meiling Bao ; Pu Li ; Hai Zhou ; Hongzhou Cai ; Qiang Cao ; Liangjun Tao ; Xiaoxin Meng ; Xiaobing Ju ; Chao Qin ; Jie Li ; Changjun Yin
• 关键词：miR-154 ; Prostate cancer ; CCND2
• 刊名：Urologic Oncology: Seminars and Original Investigations
• 出版年：January, 2014
• 年：2014
• 卷：32
• 期：1
• 页码：31.e9-31.e16
• 全文大小：1785 K

文摘

Background

Research has shown reduced expression levels of miR-154 in prostate cancer (CaP). However, the function and molecular mechanisms of miR-154 in this cancer type remains unknown.

Objective

The aims of this study were to examine the functional significance of miR-154 in CaP cells and to identify the novel molecular targets regulated by miR-154.

Materials and methods

miR-154 expression significantly decreased in primary CaP samples compared with nonmalignant samples measured by quantitative reverse transcription polymerase chain reaction. Restoration of miR-154 lowered the potential of CaP cell lines to grow and proliferate in vitro evaluated by CCK-8 assay, colony formation assay, and flow cytometry. miR-154 down-regulated the expression of CCND2 by binding to its 3鈥?untranslated region by luciferase reporter assay.

Conclusions

miR-154 plays a prominent role in CaP proliferation by suppressing CCND2, and it may provide a new approach to the treatment of CaP.