Antiplatelet activity of flavonoid and coumarin drugs

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• 作者：Cristina Zaragoz&aacute ; <sup>asup><sup> ; sup><sup>cristina.zaragoza@uah.essup> ; Jorge Monserrat<sup>bsup><sup> ; sup><sup>jorge.monserrat@uah.essup> ; Carolina Mantecó ; n<sup>asup><sup> ; sup><sup>carolina.mantecon@gmail.comsup> ; Lucinda Villaescusa<sup>asup><sup> ; sup><sup>lucinda.villaescusa@uah.essup> ; Francisco Zaragoz&aacute ; <sup>asup><sup> ; sup><sup>francisco.zaragoza@uah.essup> ; Melchor &Aacute ; lvarez‐Mon<sup>bsup><sup> ; sup><sup>csup><sup> ; sup><sup>mademons@gmail.comsup>
• 关键词：ACIs ; antiplatelet capacity index ; ADP ; adenosine diphosphate ; BS ; binding site ; CI ; calcium ionophore ; COX ; cyclooxygenase ; CVD ; cardiovascular disease ; DMSO ; dimethyl sulphoxide ; EDTA ; ethylenediaminetetraacetic acid ; FITC ; fluorescein isothiocyanate ; FSC ; forward scatter ; GPIIb/IIIa ; glycoprotein IIb/IIIa ; Mab ; monoclonal antibody ; NSAIDs ; nonsteroidal anti-inflammatory drugs ; PDMPs ; platelet-derived microparticles ; PPACK ; d-phenylalanyl-l-prolyl-l-arginine chloromethyl ketone ; SEM ; standard
• 刊名：Vascular Pharmacology
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：87
• 期：Complete
• 页码：139-149
• 全文大小：1729 K
• 卷排序：87

文摘

Polyphenols are used as phlebotonic drugs, but their mechanism of action remains unknown. Since platelet activity and platelet-endothelial cell interactions are involved in the pathogenesis of cardiovascular disease, this work examines whether different flavonoid and coumarin drugs are able to inhibit platelet aggregation. This specific case of coumarins, the antiplatelet effect is not linked with a possible interaction over blood coagulation since this effect only dicoumarols have it.The antiplatelet capacity of polyphenols was assayed using peripheral blood platelets from healthy controls. The distribution of the different platelets subsets was quantified by flow cytometry, using the calcium ionophore as a pro-aggregant. The number of GPIIb/IIIa receptors occupied by the drugs was assayed by flow cytometry using two CD61 surface fluorescein antibodies.All the polyphenols tested inhibited platelet aggregation. A percentage antiplatelet activity of 88.91 &plusmn; 7.98% was recorded for naringin, 48.43 &plusmn; 8.84% for naringenin, 53.83 &plusmn; 7.87% for esculetin, 54.65 &plusmn; 6.91% for fraxetin, and 25.75 &plusmn; 4.12% for coumarin. Naringin showed significantly greater percentage occupation of GPIIb/IIIa receptors than did naringenin (14.82 &plusmn; 0.81% vs. 3.90 &plusmn; 0.55%), and esculetin returned significantly higher values than fraxetin and coumarin (12.47 &plusmn; 0.97 vs. 7.53 &plusmn; 0.49 and 7.90 &plusmn; 0.69 respectively).All drugs show important antiplatelet activity. Naringin was the best antiplatelet compound, showing the greatest antiplatelet activity and the highest percentage binding of GPIIb/IIIa receptors. However, any of the compounds used could be used in the prevention of cardiovascular disease.