Immobilization free electrochemical biosensor for folate receptor in cancer cells based on terminal protection
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- 作者：Jiancong Nia ; b ; Qingxiang Wangb ; axiang236@126.com" class="auth_mail" title="E-mail the corresponding author ; Weiqiang Yanga ; Mengmeng Zhaoa ; Ying Zhanga ; Longhua Guoa ; Bin Qiua ; Zhenyu Lina ; zylin@fzu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Huang-Hao Yanga
- 关键词：Immobilization free ; Electrochemical biosensor ; Folate receptor ; Cancer cells ; Terminal protection
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 December 2016
- 年：2016
- 卷：86
- 期：Complete
- 页码：496-501
- 全文大小：528 K
文摘
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A simple, sensitive and selective electrochemical folate receptor (FR) biosensor in cancer cells has been proposed.
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The biosensor combines immobilization-free and the terminal protection of small molecule linked DNA.
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The proposed biosensor can detect FR with wide linear range (10 fM to 10 nM) and low detection limit (3.8 fM) were achieved.
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The developed biosensor has been applied to detect FR in HeLa cancer cells with the wide linear range (100–10000 cell/mL) and low detection limit (50 cell/mL).