Micro-aerobic conditions were applied to enhance the methane yield of wheat straw. Oxygen load, pulse frequency and micro-aeration period were the examined variables. Extended hydrolysis and solubilization of straw were achieved due to micro-aeration. The most efficient micro-aeration strategy increased the methane yield by 7.2%. AD process was susceptible to instability at oxygen loads higher than 10 mL O2/g VS.