Improved universal cloning of influenza A virus genes by LacZ伪-mediated blue/white selection
详细信息 查看全文
- 作者：Ute Wessels ; Olga Stech ; El-Sayed M. Abdelwhab ; Andreas Judel ; Thomas C. Mettenleiter ; Jü ; rgen Stech ; juergen.stech@fli.bund.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Influenza A virus ; Reverse genetics ; Universal cloning ; LacZ伪
- 刊名：Journal of Virological Methods
- 出版年：2015
- 出版时间：1 December 2015
- 年：2015
- 卷：225
- 期：Complete
- 页码：87-89
- 全文大小：661 K
文摘
- class="listitem" id="list_lis0005">
- class="label">•
Reverse genetics of influenza A viruses has been facilitated by universal cloning.
- class="label">•
We previously established target-primed plasmid amplification using ccdB.
- class="label">•
Additional introduction of the LacZ伪 fragment enables blue/white pre-screening.
- class="label">•
This improved plasmid allows elimination of bacterial colonies with empty clones.