In vitro models of axon regeneration

详细信息    查看全文

• 作者：Hassan Al-Ali^a ; Samuel R. Beckerman^a ; John L. Bixby^a ; ^b ; ^c ; ^d ; ^{jbixby@med.miami.edu} ; Vance P. Lemmon^a ; ^b ; ^c ; ^{vlemmon@med.miami.edu}
• 关键词：Axon regeneration ; Neurite outgrowth ; In vitro models ; Cell based assays ; Organotypic assays ; High content screening
• 刊名：Experimental Neurology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：287
• 期：part_P3
• 页码：423-434
• 全文大小：969 K
• 卷排序：287

文摘

A variety of in vitro models have been developed to understand the mechanisms underlying the regenerative failure of central nervous system (CNS) axons, and to guide pre-clinical development of regeneration-promoting therapeutics. These range from single-cell based assays that typically focus on molecular mechanisms to organotypic assays that aim to recapitulate in vivo behavior. By utilizing a combination of models, researchers can balance the speed, convenience, and mechanistic resolution of simpler models with the biological relevance of more complex models. This review will discuss a number of models that have been used to build our understanding of the molecular mechanisms of CNS axon regeneration.