Susceptibility of a Brazilian wild rodent isolate of Schistosoma mansoni to praziquantel in mice

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• 作者：Michele Costa-Silva^a ; ^c ; Lucas de Andrade Barros^b ; Juberlan Silva Garcia^a ; Renata Heisler Neves^b ; Rosâ ; ngela Rodrigues-Silva^b ; José ; Roberto Machado-Silva^c ; ¹ ; Arnaldo Maldonado-Jú ; nior^a ; ¹ ; ^{maldonad@ioc.fiocruz.br}
• 关键词：Praziquantel ; Susceptibility ; Schistosoma mansoni ; Non-human host ; Nectomys squamipes ; Parasitological study
• 刊名：Experimental Parasitology
• 出版年：2012
• 出版时间：April, 2012
• 年：2012
• 卷：130
• 期：4
• 页码：394-399
• 全文大小：309 K

文摘

The therapeutic effects of praziquantel (PZQ) against a Schistosoma mansoni isolate derived from Nectomys squamipes (isolate R) and a susceptible isolate (BH) were analyzed in Swiss mice by fecal egg counting, adult worm reduction and oogram pattern. Infected mice were orally administrated with 62.5 mg/kg (group 1), 125 mg/kg (group 2), 250 mg/kg (group 3) and 500 mg/kg (group 4), each dose divided over 3 days (49, 50 and 51 days after infection). The data were analyzed using one-way analysis of variance (ANOVA). In regard to isolate R, no fecal eggs were observed with 250 mg/Kg and 500 mg/kg (p < 0.05), whereas BH excretion reached zero with all doses. Mean worm burden reduction was significantly (p < 0.05) higher at the two highest concentrations, regardless of isolate. At 62.5 mg/kg, the percentage of immature eggs varied from 17 % (isolate R) to 38 % (isolate BH). At 125 mg/kg, the percentage of immature eggs varied from 20 % (isolate R) to 16 % (isolate BH). At 250 mg/kg, immature eggs dropped significantly to 1 % (isolate R) and 4 % (isolate BH). At 500 mg/kg, no immature eggs were found in isolate R, whereas in BH was 8 % . No dosage significantly (p > 0.05) affected the percentage of mature eggs, regardless of isolate. There was a large increase (p < 0.001) in the percentages of dead eggs in all treated groups of 62 % and 64 % in groups 3 and 4, respectively (isolate R). The percentage of dead eggs rose from 34 % (group 1) to 58 % (group 3) in isolate BH. Although group 4 showed lowest increase in the percentage of dead eggs (46 % ), it was higher (p < 0.001) compared to the 8 % in the control. Our findings indicate that the wild isolate from N. squamipes is susceptible to PZQ.