Evaluation of cancer cell deformability by microcavity array

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• 作者：Tomoko Yoshino ; ^{y-tomoko@cc.tuat.ac.jp} ; Tsuyoshi Tanaka ; Seita Nakamura ; Ryo Negishi ; Nozomi Shionoiri ; Masahito Hosokawa ; Tadashi Matsunaga
• 关键词：Cell deformability ; Breast cancer
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：520
• 期：Complete
• 页码：16-21
• 全文大小：1143 K
• 卷排序：520

文摘

A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.