Overexpression of tomato SlGGP-LIKE gene improves tobacco tolerance to methyl viologen-mediated oxidative stress

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• 作者：Dong-Yue Yang¹ ; Na-Na Ma¹ ; Kun-Yang Zhuang ; Shao-Bo Zhu ; Zhong-Ming Liu ; Xing-Hong Yang ; ^{xhyang@sdau.edu.cn}
• 关键词：A ; antheraxanthin ; Fv/Fm ; the maximal photochemical efficiency of PSII ; GDP ; guanosine 5&prime ; -diphosphate ; GFP ; green fluorescent protein ; GGP ; GDP-l-galactose phosphorylase ; HPLC ; high performance liquid chromatography ; MDA ; malondialdehyde ; MV ; methyl viologen ; PBS ; phosphate buffer saline ; PSII ; photosystem II ; O2&minus ; superoxide anion radical ; Pn ; net photosynthetic rate ; PFD ; photon flux density ; PVDF ; polyvinylidene fluoride ; REC ; relative electronic conductance ; ROS ; reactive oxygen
• 刊名：Journal of Plant Physiology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：209
• 期：Complete
• 页码：31-41
• 全文大小：2734 K
• 卷排序：209

文摘

Ascorbate (AsA) is very important in scavenging reactive oxygen species in plants. AsA can reduce photoinhibition by xanthophyll cycle to dissipate excess excitation energy. GGP is an important enzyme in AsA biosynthesis pathway in higher plants. In this study, we cloned a gene, SlGGP-LIKE, that has the same function but different sequence compared with SlGGP. The function of SlGGP-LIKE gene in response to oxidative stress was investigated using transgenic tobacco plants overexpressed SlGGP-LIKE under methyl viologen treatment. After oxidative stress treatment, transgenic tobacco lines exhibited higher levels of reduced AsA content and APX activity than WT plants. Under oxidative stress, transgenic tobacco plants accumulated less ROS and exhibited lower degrees of REC and MDA. Consequently, relatively higher levels of Pn, Fv/Fm, de-epoxidation status of xanthophyll cycle and D1 protein were maintained in transgenic tobacco plants. Hence, overexpression of SlGGP-LIKE gene enhances AsA biosynthesis and can alleviate the photoinhibition of PSII under oxidative stress.