Perfluorocarbon nanoemulsions with fluorescent, colloidal and magnetic properties

详细信息    查看全文

• 作者：Jelena M. Janjic^a ; ¹ ; ^{janjicj@duq.edu" class="auth_mail} ; Pin Shao^b ; ¹ ; Shaojuan Zhang^b ; ^c ; Xun Yang^d ; Sravan K. Patel^a ; Mingfeng Bai^b ; ^e ; ^f ; ^{baim@upmc.edu" class="auth_mail}
• 关键词：Fluorocarbon ; Fluorescence ; Macrophage ; Nanoparticle ; Cell viability ; Confocal microscopy
• 刊名：Biomaterials
• 出版年：June, 2014
• 年：2014
• 卷：35
• 期：18
• 页码：4958-4968
• 全文大小：3208 K

文摘

Bimodal imaging agents that combine magnetic resonance imaging (MRI) and nearinfrared (NIR) imaging formulated as nanoemulsions became increasingly popular for imaging inflammation in聽vivo. Quality of in聽vivo imaging using nanoemulsions is directly dependent on their integrity and stability. Here we report the design of nanoemulsions for bimodal imaging, where both photostability and colloidal stability are equally addressed. A highly chemically and photo stable quaterrylenediimide dye was introduced into perfluoro-15-crown-5 ether (PCE) nanoemulsions. The nanoemulsions were prepared with PCE and Miglyol 812N mixed at 1:1 v/v ratio as internal phase stabilized by non-ionic surfactants. Data shows exceptional colloidal stability demonstrated as unchanged droplet size (鈭?30聽nm) and polydispersity (<0.15) after 182 days follow up at both 4 and 25聽掳C. Nanoemulsions also sustained the exposure to mechanical and temperature stress, and prolonged exposure to light without changes in droplet size, ¹⁹F signal or fluorescence signal. No toxicity was observed in聽vitro in model inflammatory cells upon 24聽h exposure while confocal microscopy showed that nanoemulsions droplets accumulated in the cytoplasm. Overall, our data demonstrates that design of bimodal imaging agents requires consideration of stability of each imaging component and that of the nanosystem as a whole to achieve excellent imaging performance.