Molecular drivers of mitochondrial membrane proliferation in response to cold acclimation in threespine stickleback

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• 作者：Kelly Keenan ; Megan Hoffman ; Kristin Dullen ; Kristin M. O'Brien ; ¹ ; ^{kmobrien@alaska.edu}
• 关键词：AGPAT ; 1-acylglycerol 3-phosphate acyltransferase ; CDP-DAG ; cytidine diphosphodiacylglycerol ; CDS ; cytidine diphosphodiacylglycerol synthase ; CL ; cardiolipin ; DAG ; diacylglycerol ; EF-1α ; elongation factor-1 alpha ; G3P ; glycerol 3-phosphate ; GPAT ; glycerol 3-phosphate acyltransferase ; LPA ; lysophosphatidic acid ; LPAAT ; lysophosphatidic acid acyltransferase ; mTOR ; mechanistic target of rapamycin ; mtDNA ; mitochondrial DNA ; NRF-1 ; nuclear respiratory factor 1 ; PA ; phosphatidic acid ; PC ; phosphatidyl
• 刊名：Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：203
• 期：Complete
• 页码：109-114
• 全文大小：438 K
• 卷排序：203

文摘

Little is known about how the synthesis of mitochondrial phospholipids is integrated into mitochondrial biogenesis in fish or mammals. Glycerol-3-phosphate acyltransferase (GPAT; EC 2.3.1.15) catalyzes the addition of fatty acyl CoA to the sn-1 position of glycerol-3-phosphate, in what is considered the rate-limiting step in phospholipid biosynthesis. Previous studies have shown that mitochondrial volume density increases in oxidative skeletal muscle but not liver of Gasterosteus aculeatus (threespine stickleback) in response to cold acclimation. We hypothesized that maximal activity of GPAT would increase in oxidative skeletal muscle but not liver during cold acclimation, coinciding with mitochondrial biogenesis. GPAT activity was measured in liver and oxidative skeletal (pectoral adductor) muscle of threespine stickleback acclimated to 8 °C or 20 °C. In addition, mRNA levels of enzymes involved in phospholipid synthesis, including cytidine diphosphodiacylglycerol synthase-1 (CDS1), CDS2, GPAT1, GPAT2 and 1-acylglycerol 3-phosphate acyltransferase-2 (AGPAT2), were quantified in liver and pectoral muscle of stickleback harvested during cold acclimation. GPAT activity and transcript levels of AGPAT2 increased in response to cold acclimation in pectoral muscle but not liver. Transcript levels of GPAT1 increased in liver but not pectoral muscle. Overall our results suggest that the activity of GPAT, and possibly AGPAT as well, increase during cold acclimation and may contribute to mitochondrial phospholipid biosynthesis required for mitochondrial biogenesis.