Fungicidal effect of thymoquinone involves generation of oxidative stress in Candida glabrata

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• 作者：Hala Almshawit ; ^{hala.almshawit@gmail.com} ; ^{hala.almshawit@rmit.edu.au} ; Ian Macreadie
• 关键词：Thymoquinone ; Candida glabrata ; Oxidative stress ; Biofilm
• 刊名：Microbiological Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：195
• 期：Complete
• 页码：81-88
• 全文大小：1346 K
• 卷排序：195

文摘

The antifungal effect of thymoquinone, a component of black seed essential oil, has been studied on different types of fungi. Its mechanism of action as an antifungal has not been described yet. This study demonstrates the fungicidal effect of thymoquinone on different Candida species with particular emphasis on C. glabrata planktonic cells and biofilms. Since cell death was induced via the generation of oxidative stress as evidenced by the abrogation of thymoquinone toxicity in cells incubated with antioxidants, a part of thymoquinone’s mechanism of action includes a direct involvement as a pro-oxidant. This was further confirmed by measuring the generation of reactive oxygen species, glutathione level reduction and decrease in mitochondrial membrane potential. The oxidative stress caused by thymoquinone was confirmed to be the cause of death and not a result of cell death.