A new amplicon based approach of whole mitogenome sequencing for phylogenetic and phylogeographic analysis: An example of East African white-eyes (Aves, Zosteropidae)
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- 作者：Harald Meimberga ; meimberg@boku.ac.at" class="auth_mail" title="E-mail the corresponding author ; Christina Schachtlerb ; c ; Manuel Curtod ; Martin Husemanne ; Jan Christian Habelf
- 关键词：Amplicon sequencing ; Illumina ; MiSeq ; Mitogenomics ; Radiation ; Zosterops
- 刊名：Molecular Phylogenetics and Evolution
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：102
- 期：Complete
- 页码：74-85
- 全文大小：1175 K
文摘
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NGS still plays a minor role for comparative phylogenetic analyses.
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Using multiplexed PCR we cover the whole mitochondrial genome with single amplicons.
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Properties are similar to Sanger sequences and suitable for phylogenetic analysis.
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For East African white-eye bird species (Zosteropidae) we show improved resolution.