Differential expression of Low density lipoprotein Receptor-related Protein 1 (LRP-1) and matrix metalloproteinase-9 (MMP-9) in prostate gland: From normal to malignant lesions

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• 作者：Mó ; nica B. Gilardoni^a ; ^{mgilard@fcq.unc.edu.ar} ; Marí ; a M. Remedi^a ; Mabel Oviedo^b ; Tristá ; n Dellavedova^c ; Juan P. Sarrí ; a^c ; Laura Racca^c ; Mariana Dominguez^c ; Claudia G. Pellizas^a ; Ana C. Donadio^a
• 关键词：Prostate ; Cancer ; LRP-1 ; MMP-9 ; Immunohistochemistry ; Zymography
• 刊名：Pathology - Research and Practice
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：213
• 期：1
• 页码：66-71
• 全文大小：1894 K
• 卷排序：213

文摘

Metalloproteinases (MMPs) are relevant modulators of inflammation, tumor microenvironment, cancer invasion and metastasis. They can be regulated by the Low density lipoprotein Receptor–related Protein 1 (LRP-1), a receptor reported to mediate the clearance of lipoproteins, extracellular matrix (ECM) macromolecules and proteinases. The aim of this study was to evaluate the expression of LRP-1, MMP-2 and MMP-9 across various grades of prostatic diseases as benign prostatic hyperplasia (BPH), BPH plus prostatitis (BPH + P), high grade prostatic intraepithelial neoplasia (HGPIN) and prostate cancer (PCa).MethodsLRP-1 was analyzed using immunohistochemistry and MMPs proteolytic activity by zymography in prostate tissues with different prostatic diseases.ResultsLRP-1 was detected in epithelial cells in BPH (16/18), BPH + P (21/21) and HGPIN (6/6), with a staining intensity of 1+, 1+/2+ and 3+, respectively. In PCa, LRP-1 was absent in 19/27 samples while a low expression was observed in 8/27 biopsies. MMP-9 activity was higher and statistically significant in PCa than in BPH (p ≤ 0.01).ConclusionConsidering that LRP-1, by mediating the clearance of MMPs, is involved in the regulation of ECM remodeling and cell migration, we conclude that a decreased expression of LRP-1 could be involved with the increasing activity of MMPs shown in cancers.