Aqueous extract from Aconitum carmichaelii Debeaux reduces liver injury in rats via regulation of HMGB1/TLR4/NF-ΚB/caspase-3 and PCNA signaling pathways

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• 作者：Jian-Xing Luo^a ; ¹ ; ² ; ³ ; ^{863095714@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Yang Zhang^a ; ¹ ; ² ; ^{416736357@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Xiao-Yu Hu^a ; ² ; ⁴ ; ^{xiaoyuhu@aliyun.com" class="auth_mail" title="E-mail the corresponding author} ; Guo Chen^a ; ² ; ^{chenguo1978@sina.com" class="auth_mail" title="E-mail the corresponding author} ; Xi-Yun Liu^b ; ⁵ ; ^{84613791@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Hong-Ming Nie^c ; ⁶ ; ^{beining0630@126.com" class="auth_mail" title="E-mail the corresponding author} ; Jing-Li Liu^b ; ⁶ ; ^{254979859@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Da-chao Wen^b ; ⁶ ; ^{991271163@qq.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：AEACD ; aqueous extract from Aconitum carmichaelii Debeaux ; ALF ; acute liver failure ; D-GalN ; D-galactosamine ; TRL4 ; toll-like receptor 4 ; HMGB1 ; high-mobility group box 1 protein ; H&E ; hematoxylin and eosin ; NF-κB ; nuclear factor kappa B ; PCNA ; proliferating cell nuclear antigen ; SNMC ; Stronger Neo-Minophagen C ; RT-PCRs ; real-time reverse transcription polymerase chain reactions
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：13 May 2016
• 年：2016
• 卷：183
• 期：Complete
• 页码：187-192
• 全文大小：1223 K

文摘

Aconitum carmichaelii Debeaux is a well-known Chinese herb that has been used to treat liver diseases for many years in China. We investigated the effects of aqueous extract from Aconitum carmichaelii Debeaux (AEACD) on acute liver failure and identified the possible mechanisms of these effects.

Material and methods

Specific pathogen-free (SPF) male Wistar rats were used to establish acute liver failure model by intraperitoneal injection of D-galactosamine (D-GalN) and treated with Stronger Neo-Minophagen C (SNMC) and AEACD by gavage. Then, the serum biochemical parameters, the pathological scores in the liver tissue, the mRNA expressions of toll- like receptor 4 (TLR4), nuclear factor kappa B (NF-κB), high mobility group box 1 (HMGB1) and caspase-3, the proliferating cell nuclear antigen (PCNA) positive rates were analyzed.

Results

The liver function was improved, the pathological scores were decreased, the expressions the TLR4, NF-κB, HMGB1, and caspase-3 were inhibited, and the PCNA positive rates were increased by both SNMC and AEACD, but AEACD induced greater effects.

Conclusions

AEACD protected liver function by inhibiting inflammatory reaction, apoptosis and promoting liver tissue regeneration in the acute liver failure rats induced by D-galactosamine.