Broad substrate affinity and catalytic diversity of fibrinolytic enzyme from Pheretima posthumous—Purification and molecular characterization study
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- 关键词：EFE ; earthworm fibrinolytic enzyme ; CD ; circular dichroism ; BBB ; blood brain barrier ; pI ; isoelectric point ; MALDI-TOF ; matrix-assisted laser desorption/ionization- time of flight ; Maldi/MS ; matrix-assisted laser desorption/ionization/mass spectroscopy ; SK ; streptokinase ; SAK ; staphylokinase ; t-PA ; tissue plasminogen activators ; JAK/STAT ; janus kinase/signal transducers and activators of transcription ; DEAE ; diethylaminoethyl ; MWCO ; molecular weight cut off ; IEF ; isoelectric focusing ; SDS-PAGE
- 刊名：International Journal of Biological Macromolecules
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：95
- 期：Complete
- 页码：1011-1021
- 全文大小：3031 K
- 卷排序：95
文摘
In this research, a serine protease was isolated and purified from Indian earthworm Pheretima posthumous by fractionation with ammonium sulfate followed by ion exchange and size exclusion chromatography. The molecular weight of purified protease was determined 29.5 kDa by Maldi-TOF/MS. The enzyme exhibited a maximum proteolytic activity of 1.2 U/ml with specific activity of 17.65 U/mg at pH 8 and temperature 40 °C. 2D electrophoresis study illustrated purity of enzyme, purified as a single peptide and isoelectric point (pI) 4.5. The enzyme has shown tremendous stability and proteolytic activity in the wide range of pH range (4–12) and temperatures (20–60 °C). The kinetic constant Km and Vmax of purified protease were reported 0.09 mg/ml and 23.25 mg/ml/min. The enzyme also possesses excellent catalytic capacity with Kcat (341.9 min−1) and catalytic efficiency (3798.88). The N-terminal sequence of purified protease Arg-Lys-Lys-Gly-Ala-Ser-Try-Phe-Try-Pro-Trp-Ser-Val-Lys-Lys-Arg, PMF and MS/MS studies had shown a partial homology with Lumbrokinase-P2 (2) from Lumbricus rubellus. The CD spectroscopy result provided an evidence for broad substrate affinity and stability of enzyme. The different forms of secondary structures determined in EFE result broad substrate affinity of enzyme.