Crystal structure of the Saccharomyces cerevisiae monoglyceride lipase Yju3p
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- 作者：Philipp Aschauera ; Srinivasan Rengacharia ; Joerg Lichteneggera ; Matthias Schittmayerb ; c ; Krishna Mohan Padmanabha Dasa ; Nicole Mayerd ; Rolf Breinbauerd ; Ruth Birner-Gruenbergerb ; c ; Christian C. Grubera ; e ; Robert Zimmermanna ; Karl Grubera ; Monika Oberera ; m.oberer@uni-graz.at" class="auth_mail" title="E-mail the corresponding author
- 关键词：Monoglyceride lipase ; Monoacylglycerol lipase ; Crystal structure ; Lipase&ndash ; substrate-complex ; Lipase cap ; Substrate specificity
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：1861
- 期：5
- 页码：462-470
- 全文大小：2239 K
文摘
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The structures of yeast monoglyceride lipase Yju3p in its free form and in complex with a substrate-mimic were determined.
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The plasticity of the Yju3p cap enables a novel access path to the active site.
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Activity assays revealed a strong preference of Yju3p for monounsaturated monoglycerides.
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These structures correlate substrate binding pockets to substrate preference.