Isolation and characterization of a Brazilian strain of yellow fever virus from an epizootic outbreak in 2009

详细信息    查看全文

• 作者：Taissa Ricciardi Jorge^a ; ¹ ; ^{taissarj@gmail.com} ; Ana Luiza Pamplona Mosimann^a ; ¹ ; ^{anamosimann@fiocruz.br} ; Lucia de Noronha^b ; ^{lnno@terra.com.br} ; Angela Maron^c ; ^{angela.maron@gmail.com} ; Claudia Nunes Duarte dos Santos^a ; ^{clsantos@fiocruz.br}
• 关键词：Yellow fever virus ; Wild-type strain ; Brazil ; Epizootic
• 刊名：Acta Tropica
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：166
• 期：Complete
• 页码：114-120
• 全文大小：1364 K
• 卷排序：166

文摘

During a series of epizootics caused by Yellow fever virus in Brazil between 2007 and 2009, a monkey was found dead (May 2009) in a sylvatic area in the State of Paraná. Brain samples from this animal were used for immunohistochemical analysis and isolation of a wild-type strain of YFV. This viral strain was characterized, and sequence analyzes demonstrated that it is closely related with YFV strains of the recently identified subclade 1E of the South American genotype I. Further characterization included indirect-immunofluorescence of different infected cell lines and analysis of the kinetics of virus replication and infectivity inhibition by type I IFN. The generated data contributes to the knowledge of YFV evolution and phylogeny. Additionally, the reagents generated and characterized during this study, such as a panel of monoclonal antibodies, are useful tools for further studies on YFV. Lastly, this case stresses the importance of yellow fever surveillance through sentinel monkeys.