文摘
Substrate preferences of NPP1 were analyzed by combining biochemical experiments, homology modeling and docking studies. All nucleotides are hydrolyzed to nucleoside 5’-monophosphates. The mechanism of 2’,3’’-cGAMP hydrolyis by NPP1 was elucidated. The cyclic dinucleotide 2’,3’’-cGAMP yields 5’-AMP and 5’-GMP, while 3’,3’’-bridged cyclic dinucleotides are not hydrolyzed.