Pulse electric fields (PEFs) have little influence on antioxidant activity of kombucha analogues.
PEFs have minimum effect on kombucha bioactives at high feed flows.
Acid-acetic bacteria from kombucha analogues were more sensitive to PEF than yeasts.
Higher yeast inhibition was obtained at higher feed flows.
Lower specific energies produce kombucha analogues with higher phenolic and flavonoid contents.