Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

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• 作者：Dé ; bora de Souza Collares Maia Castelo-Branco¹ ; Jamille Alencar Sales² ; Raimunda Sâ ; mia Nogueira Brilhante¹ ; ^{brilhante@ufc.br" class="auth_mail" title="E-mail the corresponding author} ; Glaucia Morgana de Melo Guedes¹ ; Yago Brito de Ponte² ; Cé ; lia Maria de Souza Sampaio² ; Tereza de Jesus Pinheiro Gomes Bandeira¹ ; José ; Luciano Bezerra Moreira¹ ; Lucas Pereira de Alencar² ; Manoel de Araú ; jo Neto Paiva² ; Rossana de Aguiar Cordeiro¹ ; André ; Jalles Monteiro³ ; Waldemiro de Aquino Pereira-Neto¹ ; José ; Jú ; lio Costa Sidrim¹ ; Marcos Fá ; bio Gadelha Rocha¹ ; ²
• 关键词：Prawn ; Water ; Enterobacteria ; Vibrio ; Antibiotics
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：9
• 期：1
• 页码：27-31
• 全文大小：304 K

文摘

To investigate the isolation of enterobacteria associated with Macrobrachium amazonicum (M. amazonicum) farming and evaluate the in vitro antimicrobial susceptibility of Vibrio strains.

Methods

Strains were isolated from female M. amazonicum prawns and environmental and hatchery water. Biochemical assays were used to identify bacterial genera and those belonging to the genus Vibrio were submitted to further analyses for species identification, through Vitek 2 automated system and serotyping. Susceptibility test was performed according to Clinical Laboratory Standards Institute.

Results

The following genera of enterobacteria were recovered: Enterobacter (n = 11), Citrobacter (n = 10), Proteus (n = 2), Serratia (n = 2), Kluyvera (n = 2), Providencia (n = 2), Cedecea (n = 1), Escherichia (n = 1), Edwardsiella (n = 1) and Buttiauxella (n = 1). As for Vibrio, three species were identified: Vibrio cholerae non-O1/non-O139 (n = 4), Vibrio vulnificus (V. vulnificus) (n = 1) and Vibrio mimicus (n = 1). Vibrio spp. showed minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics, except for V. vulnificus, which presented intermediate profile to ampicillin.

Conclusions

Enterobacteria do not seem to be the most important pathogens associated with M. amazonicum farming, whereas the recovery of Vibrio spp. from larviculture, with emphasis on Vibrio cholerae and V. vulnificus, deserves special attention due to their role as potentially zoonotic aquaculture-associated pathogens. Furthermore, the intermediate susceptibility of V. vulnificus to ampicillin reflects the importance of monitoring drug use in prawn farming.