In vitro and in vivo evaluation of tissue-cultured mountain ginseng on penile erection

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• 作者：Ho Sung Lee¹ ; Young Joo Lee¹ ; Yoon Hee Chung² ; Moo Yeol Lee³ ; Sung Tae Kim¹ ; Sung Kwon Ko⁴ ; Mariko Momoi⁵ ; Yutaka Kondoh⁵ ; Fumio Sasaki⁵ ; Ji Hoon Jeong¹ ; ^{jhjeong3@cau.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：corpus cavernosum ; erectile function ; mountain ginseng ; nitric oxide ; smooth muscle
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：40
• 期：4
• 页码：334-343
• 全文大小：4136 K

文摘

Progressed tissue culture techniques have allowed us to easily obtain mass products of tissue-cultured mountain ginseng over 100 yr old (TCMG-100). We investigated the effects of TCMG-100 extract on erectile function using in vitro and in vivo studies.

Methods

To examine the relaxation effects and mechanisms of action of TCMG-100 on rabbit cavernosal strips evaluated in an organ bath. To investigate the long-term treatment effect of TCMG-100, 8-wk administration was performed. After administration of TCMG-100, intracavernosal pressure, cyclic guanosine monophosphate and nitric oxide (NO) levels of cavernosal tissue, serum testosterone level, histological observation of collagen fiber, endothelium, smooth muscle cell, and transforming growth factor-β1 were investigated.

Results

TCMG-100 extract displayed dose-dependent relaxation effects on precontracted rabbit corporal smooth muscle. The TCMG-100-induced relaxation was significantly reduced by removing the endothelium, and treatment with an NO synthase inhibitor or NO scavenger. Eight weeks of TCMG-100 administration increased intracavernosal pressure in a rat model. The levels of cyclic guanosine monophosphate and NO in the corpus callosum and serum testosterone level were also increased by TCMG-100 treatment. Furthermore, histological evaluation of collagen, smooth muscle, and endothelium showed increases in endothelium and smooth muscle, and a decrease in transforming growth factor-β1 expression.

Conclusion

These relaxation effects on corporal smooth muscle and increased erectile function suggest that TCMG-100 might be used as an alternative herbal medicine to improve erectile function.