A MALDI-MS-based quantitative glycoprofiling method on a 96-well plate platform

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• 作者：Kyoung-Jin Kim^a ; Yoon-Woo Kim^a ; Han-Gyu Park^a ; Cheol-Hwan Hwang^a ; In Young Park^b ; ^c ; Kwon-Young Choi^d ; Yung-Hun Yang^e ; Young Hwan Kim^b ; ^c ; ^f ; ^{yhkim@kbsi.re.kr} ; Yun-Gon Kim^a ; ^{ygkim@ssu.ac.kr}
• 关键词：High-throughput analysis ; MALDI-MS ; N-glycan ; UPLC-FLR ; Biopharmaceuticals
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 February 2017
• 年：2017
• 卷：46
• 期：Complete
• 页码：150-156
• 全文大小：1305 K
• 卷排序：46

文摘

High-throughput MALDI-MS based quantitative targeted glycomics (HT MALDI-MS QTaG) is a 96-well plate platform for total N-glycan analysis. HT MALDI-MS QTaG method enables to simultaneously handle a number of samples with good reproducibility. The 96-well filter plate (10-kDa cut-off) is a good alternative to the conventional chromatographic purification process. HT MALDI-QTaG method can be applied into clinical diagnosis and development of biopharmaceutical products.