Synthetic p-tetrasulphonatocalix[4]arene as novel excipient for lipase-complex

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• 作者：Irshad Ali Veesar ; Shahabuddin Memon ; Muhammad Noman Syed
• 关键词：Lipase ; Enzyme ; Excipient ; Hydrolysis ; Calixarene ; p-Nitrophenyl palmitate
• 刊名：Biochemical Engineering Journal
• 出版年：2013
• 出版时间：15 October, 2013
• 年：2013
• 卷：79
• 期：Complete
• 页码：71-76
• 全文大小：1074 K

文摘

The present article describes formation of excipient-CRL complex from water soluble calix[4]arene derivative (3 as excipient) and Candida rugosa lipase (CRL), which is proposed as a reusable form of enzyme that is free from steric and diffusion limitations associated with those enzymes immobilized onto porous solid supports. The excipient-CRL could completely hydrolyze 50 mM p-nitrophenyl palmitate (p-NPP) in Tris-HCl buffer at a wide range of temperatures, i.e. 30-80 ¡ãC. It is stable under stirred conditions and could be reused multiple times without loss of enzyme activity. It was observed that excipient-CRL complex shows a significant effect on the enzyme activity with an enhancement in thermal stability, while pH and temperature affect the activity of excipient-CRL as well as free CRL. Consequently, the excipient-CRL was found more active than free CRL for the hydrolysis of p-NPP in respect of its reusability.