The development of a multiplex real-time RT-PCR for the detection of adenovirus, astrovirus, rotavirus and sapovirus from stool samples
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- 作者：Susan Bennett ; susan.bennett@ggc.scot.nhs.uk ; Rory N. Gunson
- 关键词：PCR ; polymerase chain reaction ; Ct ; cycle threshold ; GAS ; gastroenteritis ; IID ; infectious intestinal disease
- 刊名：Journal of Virological Methods
- 出版年：2017
- 出版时间：April 2017
- 年：2017
- 卷：242
- 期：Complete
- 页码：30-34
- 全文大小：302 K
- 卷排序：242
文摘
Rapid simultaneous PCR diagnosis of viral gastroenteritis is advantageous. Many difficulties encountered when attempting to multiplex the assays. Development of a single multiplex assay for the detection of viral gastroenteritis from stool samples. The multiplex developed is sensitive, specific, robust and reproducible.