Synthesis and evaluation of fluorescence properties of Cu²⁺selective azocalix[4]arenes and their application in living cell imaging

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• 作者：Serkan Elç ; in^a ; ^{selcin@pau.edu.tr" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Hasalettin Deligö ; z^a ; ^{hdeligoz@pamukkale.edu.tr" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Asif Ali Bhatti^b ; ^d ; ^{asif_ali00083@yahoo.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Mehmet Oguz^b ; ^{m.oguz2011@gmail.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Serdar Karakurt^c ; ^{kserdar1@yahoo.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Mustafa Yilmaz^b ; ^{myilmaz42@gmail.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Azocalixarene ; Fluorescence ; Complexation ; Binding constant ; Living cell
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：29 October 2016
• 年：2016
• 卷：234
• 期：Complete
• 页码：345-352
• 全文大小：2404 K

文摘

The present article deals with the detailed complexation study along with exploration of possible utilization of 25,26,27,28-tetra-hydroxy-11,23-di-(tert-butyl)-5,17-di-(2-antracenyl)azocalix[4]arene(7) and 25,26,27,28-tetra-hydroxy-11,23-di-(tert-butyl)-5,17-di-(1-pyreneyl)azocalix[4]arene (8) as bio-imaging fluorescent probe to detect Cu²⁺ in human colon cell line, SW-620, observed by fluorescence microscopy. Complexation properties of ligands 7 and 8 toward selected transition metal ions have been investigated by UV–vis and fluorescence spectroscopies. It has been noticed that ligands 7 and 8 are not only proved to be an efficient Cu²⁺selective chromoionophore but also possess effective fluorescence properties by complexing with Cu²⁺ ions. The FTIR spectroscopic method has also been applied for further confirmation of the complexation phenomenon of ligands 7 and 8 with metal ion and were found to be adequate. Moreover, fluorescence microscopy studies showed that ligand 7 may be used as fluorescent probe to detect Cu²⁺ in living cells.