Peptide nucleic acid probe-based fluorescence melting curve analysis for rapid screening of common JAK2, MPL, and CALR mutations

详细信息    查看全文

• 作者：Joonhong Park^a ; ^b ; ¹ ; Minsik Song^c ; ¹ ; Woori Jang^a ; ^b ; Hyojin Chae^a ; ^b ; Gun Dong Lee^b ; KyungTak Kim^c ; Heekyung Park^c ; Myungshin Kim^a ; ^b ; ^{microkim@catholic.ac.kr} ; Yonggoo Kim^a ; ^b ; ^{yonggoo@catholic.ac.kr}
• 关键词：Peptide nucleic acid ; FMCA ; Mutation screening ; JAK2 ; MPL ; CALR
• 刊名：Clinica Chimica Acta
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：465
• 期：Complete
• 页码：82-90
• 全文大小：1495 K
• 卷排序：465

文摘

Detection of JAK2, MPL, and CALR mutations is essential in BCR-ABL1-negative MPN. Peptide nucleic acid-based fluorescence melting curve analysis is developed. The limit of detection (LOD) was approximately 10% for each mutation. Interference reactions using mixtures of different mutations were not observed. The rapid and simple PNA-based FMCA is a sensitive and specific technique.