Differentiating rapid- and slow-growing mycobacteria by difference in time to growth detection in liquid media

详细信息    查看全文

• 作者：Chung-Jong Kim^a ; Nak-Hyun Kim^a ; Kyoung-Ho Song^a ; Pyoeng Gyun Choe^a ; Eu Suk Kim^a ; Sang Won Park^a ; Hong-Bin Kim^a ; Nam-Joong Kim^a ; Eui-Chong Kim^b ; Wan Beom Park^a ; ^{wbpark1@snu.ac.kr} ; Myoung-don Oh^a
• 关键词：Atypical mycobacteria ; Time to growth detection ; Rapid-growing mycobacteria ; Slow-growing mycobacteria ; Acid-fast bacilli
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：75
• 期：1
• 页码：73-76
• 全文大小：193 K

文摘

Nontuberculous mycobacteria (NTM) are classified into 2 categories: slow-growing mycobacteria (SGM) and rapid-growing mycobacteria (RGM), based on interval to colony formation by subculture on solid media. However, little is known about the growth rate of NTM in liquid broth media. We evaluated the differences in time to growth detection (TGD) of RGM and SGM in liquid broth media according to acid-fast stain. Among the 696 NTM isolates, 201 were RGM and 495 were SGM. In acid-fast bacilli (AFB)-negative specimens, the mean TGD was 133 h for RGM and 269 h for SGM (P < 0.001). In AFB-positive specimens, the mean TGD was 112 ¡À 37 h for RGM and 155 ¡À 125 h for SGM (P = 0.063). In the AFB-negative group, a cut-off value of 6 days was most effective for distinguishing SGM from RGM; however, in the AFB-positive group, an appropriate cut-off value was hard to define with TGD only.