The role of bestrophin in airway epithelial ion transport

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• 作者：Duta ; Valentin ; Szkotak ; Artur J. ; Nahirney ; Drew ; Duszyk ; Marek
• 关键词：Small interfering RNA ; Basolateral Cl^&minus ; channel ; cGMP
• 刊名：FEBS Letters
• 出版年：2004
• 出版时间：November 19, 2004
• 年：2004
• 卷：577
• 期：3
• 页码：551-554
• 全文大小：129 K

文摘

The purpose of this study was to identify Cl⁻ channels in the basolateral membrane of airway epithelial cells at the molecular level. We have focused on a new family of Cl⁻ channels, bestrophins, which have previously been identified in retinal pigment epithelium. RT-PCR, Western blot and confocal microscopy studies revealed the presence of bestrophin in airway epithelial cells. Decreasing bestrophin expression using siRNA resulted in diminished ³⁶Cl⁻ flux. These studies also showed that bestrophin regulation is similar to that of native basolateral Cl⁻ channels. The data indicate that the presence of a functional bestrophin may contribute to the basolateral cell conductance in airway epithelial cells.