Detection of adenosine 5鈥?triphosphate by fluorescence variation of oligonucleotide-templated silver nanoclusters

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• 作者：Jennifer Daneen Lee^a ; Jinshun Cang^b ; Ying-Chieh Chen^a ; Wei-Yu Chen^a ; Chung-Mao Ou^a ; Huan-Tsung Chang^a ; ^{changht@ntu.edu.tw" class="auth_mail}
• 关键词：Ag nanoclusters ; DNA structure ; Adenosine 5&prime ; -triphosphate ; Fluorescence
• 刊名：Biosensors and Bioelectronics
• 出版年：15 August, 2014
• 年：2014
• 卷：58
• 期：Complete
• 页码：266-271
• 全文大小：724 K

文摘

Oligonucleotide-templated Ag nanoclusters (DNA-Ag NCs) prepared from AgNO₃ using an oligonucleotide (5鈥?TAACCCCTAACCCCT-3鈥? as a template and NaBH₄ as a reducing agent have been used for sensing of adenosine 5鈥?triphosphate (ATP). The fluorescence intensity and emission wavelength of DNA-Ag NCs are dependent on the pH value and ATP concentration. At pH 3.0 and 11.0, ATP shows greater effects on fluorescence of the DNA-Ag NCs. Upon increasing ATP concentration from 10 to 50 渭M, their emission wavelength at pH 3.0 shifts from 525 to 585 nm. At pH 11.0, their fluorescence intensity (510 nm) increases upon increasing ATP concentration. The circular dichroism (CD), electrospray ionization-mass spectrometry (ESI-MS), absorption, and fluorescence results indicate that ATP and pH affect the interactions between DNAs and Ag atoms, resulting in changes in their fluorescence. The DNA-Ag NCs allow detection of ATP over a concentration range of 0.1-10 渭M, with a limit of detection 33 nM. Practicality of the DNA-Ag NCs probe has been validated with the determination of ATP concentrations in the lysate of MDA-MB-231 breast carcinoma cells.