Construction of a triglyceride amperometric biosensor based on chitosan¨CZnO nanocomposite film

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• 作者：Jagriti  ; Narang ; C.S.  ; Pundir  ; ;   ; ^{pundircs@rediffmail.com} ;   ; ^{pundircs@gmail.com} ;   ; ^{pundircs@yahoo.com}
• 关键词：Triglyceride ; Amperometric biosensor ; ZnONPs ; Lipase ; Glycerol ; Kinase ; Glycerol-3-phosphate oxidase
• 刊名：International Journal of Biological Macromolecules
• 出版年：2011
• 出版时间：1 November 2011
• 年：2011
• 卷：49
• 期：4
• 页码：707-715
• 全文大小：1271 K

文摘

A method is described for construction of a novel amperometric triglyceride (TG) biosensor based on covalent co-immobilization of lipase, glycerol kinase (GK) and glycerol-3-phosphate oxidase (GPO) onto chitosan (CHIT) and zinc oxide nanoparticles (ZnONPs) composite film deposited on the surface of Pt electrode. The enzymes¨CZnONPs¨CCHIT composite was characterized by X-ray diffraction (XRD), scanning electron microscope (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The sensor showed optimum response within 6 s at pH 7.5 and temperature of 35 ¡ãC. The sensor measures current due to electrons generated at 0.4 V against Ag/AgCl from H₂O₂, which is produced from triolein by co-immobilized enzymes. A linear relationship was obtained between a wide triolein concentration range (50?50 mg/dl) and current (mA) under optimum conditions. The biosensor showed high sensitivity, low detection limit (20 mg/dl) and good storage stability (half-life of 7 months at 4 ¡ãC). The biosensor was unaffected modified by a number of serum substances at their physiological concentrations. The biosensor was evaluated and employed for determination of TG in sera in apparently healthy subjects and persons suffering from hypertriglyceridemia.