Chitosan/sulfated locust bean gum nanoparticles: In vitro and in vivo evaluation towards an application in oral immunization

详细信息    查看全文

• 作者：Luis Braz^a ; ^b ; ^c ; ^d ; ^{lvbraz@ualg.pt} ; Ana Grenha^d ; ^e ; Domingos Ferreira^f ; Ana M. Rosa da Costa^a ; Carlos Gamazo^g ; Bruno Sarmento^h ; ⁱ ; ^j ; ^{bruno.sarmento@ineb.up.pt}
• 关键词：Locust bean gum ; Oral immunization ; Polymeric nanoparticles
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：786-797
• 全文大小：1439 K
• 卷排序：96

文摘

This work proposes the design of nanoparticles based on locus bean gum (LBG) and chitosan to be used as oral immunoadjuvant for vaccination purposes. LBG-based nanoparticles were prepared by mild polyelectrolyte complexation between chitosan (CS) and a synthesized LBG sulfate derivative (LBGS). Morphological characterization suggested that nanoparticles present a solid and compact structure with spherical-like shape. Sizes around 180–200 nm and a positive surface charge between +9 mV and +14 mV were obtained. CS/LBGS nanoparticles did not affect cell viability of Caco-2 cells after 3 h and 24 h of exposure when tested at concentrations up to 1.0 mg/mL. Two model antigens (a particulate acellular extract HE of Salmonella enterica serovar Enteritidis, and ovalbumin as soluble antigen) were associated to CS/LBGS nanoparticles with efficiencies around 26% for ovalbumin and 32% for HE, which resulted in loading capacities up to 12%. The process did not affect the antigenicity of the associated antigens. BALB/c mice were orally immunized with ovalbumin-loaded nanoparticles (100 μg), and results indicate an adjuvant effect of the CS/LBGS nanoparticles, eliciting a balanced Th1/Th2 immune response. Thus, CS/LBGS nanoparticles are promising as antigen mucosal delivery strategy, with particular interest for oral administration.