Silver nanoparticles: Significance of physicochemical properties and assay interference on the interpretation of in vitro cytotoxicity studies

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• 作者：Kausar B. Riaz Ahmed ; Amber M. Nagy ; Ronald P. Brown ; Qin Zhang ; Subhas G. Malghan ; Peter L. Goering ; ^{peter.goering@fda.hhs.gov}
• 关键词：AgNPs ; silver nanoparticles ; nAg ; nanosilver ; NP ; nanoparticle ; AuNP ; gold nanoparticle ; ENM ; engineered nanomaterial ; DE ; dual energy ; TEM ; transmission electron microscopy ; AAS ; atomic absorption spectroscopy ; ROS ; reactive oxygen species ; SOD ; superoxide dismutase ; COX-2 ; cyclooxygenase-2 ; Ctr1 ; copper transporter 1 ; AO/EtBr ; acridine orange ethidium bromide ; DNA ; deoxyribonucleic acid ; EC50 ; effective concentration 50 ; ENM ; engineered nanomaterial ; GSH ; glutathione ; IL ; interleukin ; JNK ; jun
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：38
• 期：Complete
• 页码：179-192
• 全文大小：973 K
• 卷排序：38

文摘

Silver nanoparticles (AgNPs) have generated a great deal of interest in the research, consumer product, and medical product communities due to their antimicrobial and anti-biofouling properties. However, in addition to their antimicrobial action, concerns have been expressed about the potential adverse human health effects of AgNPs. In vitro cytotoxicity studies often are used to characterize the biological response to AgNPs and the results of these studies may be used to identify hazards associated with exposure to AgNPs. Various factors, such as nanomaterial size (diameter), surface area, surface charge, redox potential, surface functionalization, and composition play a role in the development of toxicity in in vitro test systems. In addition, the interference of AgNPs with in vitro cytotoxicity assays may result in false negative or false positive results in some in vitro biological tests. The goal of this review is to: 1) summarize the impact of physical-chemical parameters, including size, shape, surface chemistry and aggregate formation on the in vitro cytotoxic effects of AgNPs; and 2) explore the nature of AgNPs interference in in vitro cytotoxicity assays.