A maleimide-based thiol fluorescent probe and its application for bioimaging

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• 作者：Lijun Qu^a ; ¹Author Vitae ; Caixia Yin^a ; ^{yincx@sxu.edu.cn" class="auth_mail}Author Vitae ; Fangjun Huo^b ; ¹ ; ^{huofj@sxu.edu.cn" class="auth_mail}Author Vitae ; Jianfang Li^aAuthor Vitae ; Jianbin Chao^bAuthor Vitae ; Yongbin Zhang^bAuthor Vitae
• 关键词：1 ; 8-Diamino naphthalene ; Thiol ; Fluorescent probe ; Bioimaging
• 刊名：Sensors and Actuators B: Chemical
• 出版年：May, 2014
• 年：2014
• 卷：195
• 期：Complete
• 页码：246-251
• 全文大小：1375 K

文摘

A novel thiol-reactive fluorescence probe based on the 1,8-naphthalic anhydride fluorophore was designed and synthesized that selectively detected thiols over other relevant biological species by the turning on of its fluorescence through a Michael addition reaction. In aqueous buffer solutions at physiological pH, the probe exhibited 4-fold enhancement in fluorescence intensity by a Michael addition of Cys, Hcy, and GSH to the maleimide appended to a naphthalic. Cell permeability and live cell imaging of thiols are also demonstrated.