Assessment of sanitation efficacy against Escherichia coli O157:H7 by rapid measurement of intracellular oxidative stress, membrane damage or glucose active uptake
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- 作者：Andrea Cossua ; 1 ; Phuong Leb ; 1 ; Glenn M. Younga ; Nitin Nitina ; b ; nnitin@ucdavis.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Rapid measurement ; Oxidative stress ; Membrane damage ; Glucose uptake ; Escherichia coli O157 ; H7
- 刊名：Food Control
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：71
- 期：Complete
- 页码：293-300
- 全文大小：1022 K
文摘
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Oxidative stress by NaOCl or H2O2 in Escherichia coli O157:H7 was measured with APF or H2DCFDA.
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Membrane damage and glucose uptake were measured using PI and 2-NBDG.
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20">Oxidative stress probes did not show significant correlation with microbial reduction.
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25">PI permeation had significant correlation with microbial reduction when NaOCl was used.
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Measurement of 2-NBDG uptake was shown as the best surrogate for plate counting.