Identification of distinctive interdomain interactions among ZP-N, ZP-C and other domains of zona pellucida glycoproteins underlying association of chicken egg-coat matrix
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- 作者：Hiroki Okumuraa ; hokumura@meijo-u.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Takahiro Satoa ; Rio Sakumaa ; Hideaki Fukushimaa ; Tsukasa Matsudab ; Minoru Ujitaa
- 关键词：ZP ; zona pellucida ; IHP ; internal hydrophobic patch ; EHP ; external hydrophobic patch ; TGFR ; transforming growth factor-β receptor ; Trx ; thioredoxin ; His6 ; hexahistidine ; EGF ; epidermal growth factor ; DTT ; dithiothreitol ; CBB ; Coomassie Brilliant Blue ; RT ; room temperature ; DIC ; differential interference contrast ; MBP ; maltose binding protein ; THP ; Tamm&ndash ; Horsfall protein
- 刊名：FEBS Open Bio
- 出版年：2015
- 出版时间：2015
- 年：2015
- 卷：5
- 期：Complete
- 页码：454-465
- 全文大小：2061 K
文摘
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Chicken ZP1 and ZP3 assemble through strong interactions between their ZP-C domains.
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ZP-C domains of chicken ZP1 and ZP3 are deeply embedded in the egg-coat matrix.
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Chicken ZP1 forms a homocomplex through non-covalent interaction between repeat domains.
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Chicken ZPD is deposited on the interstices of ZP1–ZP3 matrix in the egg coat.
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We propose a model for the architecture of chicken egg-coat matrix from these results.