N-alkylamide profiling of Achillea ptarmica and Achillea millefolium extracts by liquid and gas chromatography-mass spectrometry

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• 作者：Lieselotte Veryser^a ; Lien Taevernier^a ; Evelien Wynendaele^a ; Yannick Verheust^b ; Ann Dumoulin^b ; Bart De Spiegeleer^a ; ^{Bart.DeSpiegeleer@UGent.be}
• 关键词：N-alkylamides ; Achillea millefolium ; Achillea ptarmica ; HPLC&ndash ; ESI&ndash ; MS ; GC&ndash ; EI&ndash ; MS
• 刊名：Journal of Pharmaceutical Analysis
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：7
• 期：1
• 页码：34-47
• 全文大小：1642 K
• 卷排序：7

文摘

Achillea millefolium and Achillea ptarmica are both plants belonging to the Asteracea family and are traditionally used for their medicinal properties. It has already been shown that some N-alkylamides (NAAs) are responsible for these pharmacological actions. Therefore, in the present study, the NAA content of the two plants was analytically characterised. Different extracts were prepared from the roots, the leaves, the stems and the flowers. The structures of NAAs have been assigned in ethanolic extracts of Achillea millefolium and Achillea ptarmica using high performance liquid chromatography – electrospray ionisation – mass spectrometry (HPLC–ESI–MS) and gas chromatography – electron impact – mass spectrometry (GC–EI–MS). Using both analytical techniques, the structures of 14 and 15 NAAs have been assigned in Achillea ptarmica and Achillea millefolium, respectively. Structures of two new NAAs, previously never observed in Achillea ptarmica, were assigned: deca-2E,6Z,8E-trienoic acid 2-methylbutylamide (homospilanthol) or a related isomeric compound and deca-2E,4E-dienoic acid N-methyl isobutylamide. The structure of homospilanthol or a related isomeric compound was also assigned in Achillea millefolium for the first time.