Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties

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• 作者：Michael Eichenberger^a ; ^b ; Beata Joanna Lehka^c ; ^d ; Christophe Folly^a ; David Fischer^a ; Stefan Martens^e ; Ernesto Simó ; n^a ; Michael Naesby^a ; ^{michaeln@evolva.com}
• 关键词：CHI ; chalcone isomerase ; CHS ; chalcone synthase ; CH3H ; chalcone 3-hydroxylase ; CPR ; cytochrome P450 reductase ; CYP ; cytochrome P450 ; C4H ; cinnamate 4-hydroxylase ; DBR ; double bond reductase ; DHC ; dihydrochalcone ; EMA ; European Medicine Agency ; FDA ; Food and Drug Administration ; F3H ; flavanone 3β-hydroxylase ; F3&prime ; H ; flavonoid 3&prime ; -hydroxylase ; FLS ; flavonol synthase ; HRT ; homologous recombination tag ; NDC ; naringin dihydrochalcone ; NHDC ; neohesperidin dihydrochalcone ; OD600 ; optical dens
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：80-89
• 全文大小：606 K
• 卷排序：39

文摘

De novo biosynthesis of phloretin in S. cerevisiae. De novo pathway extended to various dihydrochalcones of commercial interest. A barley CHS exhibits very high specificity for phloretin production.